PCR DNA Extraction Primer design

Hi, i am a genetic counsellor and i can really help you with designing primers for PCR. I have worked in lab doing DNA extraction and designing primers

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I am Medical researcher(Ph.D.), have a skills for; Molecular Biology: DNA, RNA Isolation and Purification, Cloning and Subcloning Techniques, PCR, RT-PCR, qPCR and SDS-PAGE Western Blotting Cell Biology: Establishment of stable cell lines, Knockdown by siRNA, Transfection and Lentiviral Transduction. I can help you designing PCR primers for a DNA extraction project within 7 days.

As a scientific consultant with a molecular biology background, I have extensive experience in PCR primer design and DNA extraction projects. I have a strong understanding of gene and region-specific PCR primer design techniques and have successfully designed primers for various organisms and genes of interest. I have a deep knowledge of molecular biology techniques and a thorough understanding of the principles of PCR. This allows me to design primers that are specific to the target genes or regions of interest, ensuring accurate and reliable amplification. I know how to use bioinformatic tools and databases, such as NCBI, to identify target genes and retrieve relevant sequence information. I can efficiently analyze the sequences to design primers with optimal melting temperatures, appropriate lengths, and minimal secondary structures or primer-dimers. I have a keen attention to detail and can ensure that the primer sequences meet the specific requirements outlined in the brief. I am familiar with the different types of primers, and as such it would be useful for me to know which synthesis company you intend to place a purchase order with. I am committed to delivering high-quality results in a timely manner. I understand the importance of meeting deadlines and can efficiently manage my time to ensure timely completion of the PCR primer design.

Greetings from San Diego! I believe I can help you with this project. I am a molecular biologist with PhD from UCLA and lots of experience designing primers for PCR. To get started I would just need a few more experimental details and to define the scope of the project. If you are interested, please contact me directly. Thank you!

I'm a PhD graduate in molecular biology with expertise in RNA biology. My whole thesis revolved around validation of candidate genes by qRT PCR which requires primer designing. I also have experience in R programming and bioinformatics, which would be used to find the region of interest from the reference genome. The primer sequences will be delivered along with all the details such as the GC content, presence of secondary structures, the melting temperature for all the commercial polymerase. Replies within one hour.

PhD candidate currently working with pcr based techniques and cell culture based assays. Work can be delivered timeously and accurately

I am excited to express my interest in your PCR primer design project for animal DNA extraction. With my more than 10 years expertise in this field and a proven track record in gene and region-specific PCR primer design, I am confident that I am the ideal candidate to meet your project's requirements. I believe that effective communication is key to project success. I am open to discussions and feedback throughout the project's duration. I am proficient in using communication tools such as Zoom/Teams to keep you updated on progress and address any concerns promptly.

I want to know about the target organism and start to sequence the target gene and clone by PCR , I work in the national research center in Egypt.