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I need help analyzing spatial transcriptomics data from three lung biopsies of patients with a specific lung disease. The analysis should include: - Differential expression analysis - Cell type identification - Spatial patterning analysis I have specific cell markers and reference datasets that I would like to use. Please use Seurat for this analysis. Ideal Skills and Experience: - Expertise in spatial transcriptomics - Proficiency in Seurat - Experience with differential expression, cell type identification, and spatial patterning analysis - Strong background in bioinformatics and transcriptomics
Project ID: 40176300
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32 freelancers are bidding on average $533 USD for this job

Hello, "As a team at Live Experts LLC., my colleagues and I bring in-depth expertise in bioinformatics and transcriptomics, making us well-equipped to handle your Spatial Transcriptomics Data Analysis project. We are experienced biostatisticians who have worked with a wide range of powerful tools and methods including the Seurat package - an integral part of your project requirements. With strong foundations in data science, we are skilled in conducting differential expression analysis, cell type identification, as well as spatial patterning analysis - all key facets of your project. Our familiarity with R programming language also positions us perfectly for this job, given R's dominance in transcriptomic analysis. We understand that your project is about patients with a specific lung disease, so you can trust our competent hands to ensure ethical handling and complete confidentiality of the data involved. Beyond just executing tasks, we strive to deliver insights and value through our analyses - leveraging our statistical prowess to not just report results but provide you with meaningful interpretations and recommendations based on the outcome of our analysis. At Live Experts LLC., we don't just promise to satisfy; we endeavor to exceed expectations. We are communicative team players who believe in tailoring our services to meet your specific needs. With our unwavering commitment to quality, timely delivery, and client satisfactio Thanks!
$3,500 USD in 18 days
8.4
8.4

Hi there, I understand the importance of accurately analyzing spatial transcriptomics data for your lung biopsy samples, especially given the specific disease context. With a strong background in bioinformatics and a proven track record in using Seurat for similar projects, I am well-equipped to conduct the differential expression analysis, cell type identification, and spatial patterning analysis that you require. I have extensive experience working with cell markers and reference datasets, ensuring that your specific needs will be met thoroughly. I will ensure that the analysis not only meets your expectations but also provides insightful interpretations to enhance your research outcomes. I propose to complete this analysis in 7 days, allowing sufficient time for comprehensive exploration of your data. Thanks,
$450 USD in 2 days
5.5
5.5

Hi i am an experienced regression analyst in python/Matlab with PhD in applied mathematics and data analyst.I can help you regression analysis of your data in Matlab/python coding, optimization, numerical analysis،simulation and stock data prediction of the stock data.
$500 USD in 3 days
5.5
5.5

Hello, I understand you’re looking for a comprehensive spatial transcriptomics analysis of lung biopsy samples, focusing on differential expression, cell type identification, and spatial patterning. I will leverage Seurat to integrate your datasets with the reference markers you provide, ensuring accurate clustering, annotation, and identification of relevant cell populations. Each analysis step will be reproducible and clearly documented. The workflow will include rigorous differential expression analysis, highlighting genes enriched in specific regions or cell types, and spatial patterning analysis to map cellular interactions and tissue architecture. Visualizations will be generated to clearly represent spatial distributions and cell type compositions, suitable for publication or downstream interpretation. With experience in bioinformatics, transcriptomics, and high-dimensional data analysis, I will ensure the results are robust, reproducible, and aligned with your research objectives. Deliverables will include processed Seurat objects, analytical scripts, and detailed reports summarizing findings, visualizations, and interpretations. Thanks, Asif
$750 USD in 5 days
5.1
5.1

Hello. I read your requirement i will do that. Please come on chat we will discuss more about this. I will waiting your reply
$251 USD in 1 day
5.0
5.0

Affordable, Early Delivery. ★★★★★★★★★★★★★★I hold a Masters degree which gives me the requisite background to handle writing from various subjects. I am a highly committed person towards my work. You can rely on QualityXenter for quality and consistency in writing. We never violate copyright rules. I have vast amount of experience in this industry since I am working from 2015 as a professional writer. I provide many modifications till to get your satisfactions. I have access to enough journals to use in your research project. I always produce quality work at VERY LOW RATES so, don't worry if you have a low budget for your work, I will be very happy to make a new client like you. I am producing quality work for my clients including ARTICLE WRITING, REPORT WRITING, ESSAY WRITING, RESEARCH PAPERS, BUSINESS PLAN, TECHNICAL WRITING, MATLAB, THESIS, ACCOUNTING & FINANCE work ETC. Go through my profile link https://www.freelancer.com/u/qualityxenter
$250 USD in 1 day
4.7
4.7

Hello, I have read the outline of your project ("Spatial Transcriptomics Data Analysis"), and I’m sure can solve the task, provide correct result, free revision guarantee. I’m an expert in various model regression, differential expression, cell type identification, spatial patterning analysis, Biostatistics, report in Rmarkdown file html/pdf/word, complete with the charts. My background is in statistics and applied mathematics using R programming for model statistics, predictive analytics, machine learning and artificial intelligence. Please share your data, I'm available, discuss detailed requirements, budget/time negotiable. Thank you. Best rgds, Bambangpe
$250 USD in 2 days
4.5
4.5

Hello Zach, I’m a senior bioinformatics / transcriptomics engineer with hands-on experience analyzing 10x Visium & spatial RNA-seq datasets using Seurat v4/v5 for disease profiling. I can share demo Seurat pipelines + annotated notebooks before we finalize. What I’ll deliver (end-to-end Seurat workflow) ✅ QC, normalization & integration of 3 biopsies ✅ Differential Expression (FindMarkers / MAST / Wilcoxon) ✅ Cell type identification using your markers + reference mapping (Azimuth / SingleR) ✅ Spatial patterning & niche analysis (SpatialFeaturePlot, Moran’s I, Hotspot) ✅ Publication-ready UMAPs, spatial maps, heatmaps & dot plots ✅ Fully reproducible R scripts + commented Seurat object Techniques I use • SCTransform + batch correction • Marker-guided + reference-based annotation • Spatial DE & co-localization analysis • Ligand-receptor & micro-environment scoring (optional) Relevant Projects ? Idiopathic Pulmonary Fibrosis – Spatial DE Mapping (Seurat) ? Tumor Microenvironment Spatial Cell Typing (10x Visium) ? Inflammatory Lung Biopsy Atlas Construction Why me ⭐ 8+ years in transcriptomics & spatial omics ⭐ Strong lung / immune cell annotation background ⭐ Clean, Git-ready code + clear biological interpretation ⚡ I can start immediately and show live Seurat demo code + spatial plots from similar lung datasets. Let’s discuss your markers, references & timelines and lock this in today.
$1,000 USD in 15 days
4.3
4.3

Hello there, With extensive experience in statistical and predictive analytics, My proficiency in SPSS combined with my programming skills in Python will not only ensure an effective data analysis but also allow for flexibility and automation, optimizing efficiency throughout the process. Throughout my career, I have consistently proven my problem-solving mindset transforming complex datasets into clear and actionable insights that clients can use to drive their business forward. Data cleaning and transformation is an often-overlooked area of importance; however, it's where precise analysis begins. My vast experience in this area ensures thorough sorting of your data for accurate analysis - leaving no stone unturned. Lastly, I recognize that clear communication is vital to successful project completion. I assure you of my commitment in communicating regularly and effectively throughout this project, leaving no room for ambiguities. Trusting me with this project will not just avail you of a qualified individual but also someone who is dedicated in leveraging their knowledge and experience for unparalleled client satisfaction.
$250 USD in 2 days
4.1
4.1

As an Electrical Engineer with a deep-rooted passion for data analysis and a master's degree in Embedded Systems, I am excited to apply my broad skill set to the unique project of spatial transcriptomics. While my core experience lies in microcontrollers and firmware development, I also possess strong capabilities in data analytics that I have applied successfully across diverse domains including healthcare. My proficiency in Seurat aligns perfectly with your requirement, ensuring the accuracy and reliability of the analysis. Notably, my comprehensive understanding of PCB design, RF hardware, and biomedical instrumentation provides me with a robust foundation that is particularly crucial for projects like yours which involve complex data generation and analysis. My skills integrate multiple domains seamlessly, from intelligent algorithm implementation for edge devices to AI-driven analytics for precise control. Ultimately, my aim is to deliver insights that drive informed decision-making, and this resonates wholly with the objectives of your project. Nurtured by years of engineering practice, I am certain that the analytical approach I bring forth will effectively solve complex biological problems such as differential expression analysis, cell-type identification and spatial patterning analysis. Trust me to provide comprehensive results while bridging the gap between rigorous bioinformatics analysis and your specific needs. Let's team up!
$750 USD in 7 days
3.9
3.9

Hello there, As an experienced researcher and data scientist, data analyst, my qualitative analysis skills perfectly align with your job requirements. My profound knowledge of Python and R Studio guarantees fast learning and adaptation to new tools. Moreover, my advanced skills in Excel make me highly competent in handling large datasets efficiently—making me proficient in extracting the best insights from your transcripts. I fully comprehend the importance of working papers and meticulously preparing financial statements, especially within strict timelines. my sharp analytical skills and extensive knowledge of excel ensure that I leave no stone unturned in making sure every detail is covered under evaluation. My passion for quality, originality and meeting deadlines makes me an excellent choice for this project. I cannot wait to prove my extensive skills to you through providing actionable insights that will help guide your decision making regarding domestic charter flights. Best Regards
$250 USD in 2 days
3.9
3.9

Hello Zach, Thank you for outlining your project so clearly. I understand you need comprehensive analysis of spatial transcriptomics data from three lung biopsies, focusing on differential expression, cell type identification, and spatial patterning, all using Seurat and your specified markers and references. My background in bioinformatics and spatial transcriptomics aligns closely with your requirements. I have extensive experience working with Seurat for single-cell and spatial data, including differential gene expression, robust cell type annotation using curated markers, and advanced spatial pattern analysis. I have supported similar projects involving lung disease, leveraging both internal and reference datasets to extract meaningful biological insights. For your project, I propose a stepwise workflow: first, rigorous quality control and normalization of your spatial transcriptomics data; followed by integration of your reference datasets and cell markers for accurate cell type identification. Differential expression analysis will be performed to highlight disease-relevant genes and pathways. Finally, I’ll apply spatial statistics and visualization techniques to map gene expression patterns and cell distributions within tissue context. Throughout, I will ensure reproducibility and clarity in reporting, with clearly annotated code and visualizations tailored to your scientific questions. I am committed to delivering actionable insights and high-quality visualizations, and am happy to discuss your preferred analysis details or any specific hypotheses you wish to address. I look forward to collaborating with you on this exciting project.
$500 USD in 2 days
2.4
2.4

As an accomplished bioinformatics company, CHARONDAJ has extensive expertise in spatial transcriptomics analysis using tools such as Seurat which aligns perfectly with your project requirements. Our team understands the significance of identifying differential expression, cell types, and spatial patterning in understanding complex biological systems. We have worked on multiple similar projects, analyzing tissue data to extract meaningful insights. What makes us well-suited for your project is our strong background in integrating various datasets, including applying specific markers and reference datasets like you've mentioned. We understand the nuances of different lung diseases, which will enable us to conduct a meticulous analysis tailored to your specific context. In sum, rely on CHARONDAJ to not only perform a comprehensive analysis with robust statistical methods but also present the results in an easily interpretable form. With our skills and experience combined with your valuable data, we can produce actionable insights that could revolutionize understanding and treatment strategies for this lung disease. Let's start extracting vital information from your precious lung biopsy data together!
$250 USD in 2 days
0.0
0.0

Hi! Greetings, Having carefully reviewed your project description, I am confident in my ability to execute this project to perfection. I possess a broad spectrum of skills, knowledge, and experience in this specific field, making me the ideal candidate to handle your project. My proficiency includes Biostatistics, Matlab and Mathematica, Data Analysis, Statistical Analysis, Statistics, Bioinformatics, Data Visualization and Data Science, which positions me as the best choice for the successful completion of your project. While I am well-prepared to begin, I have a few clarifying questions. Kindly drop me a message in the chat so that we can engage in a discussion regarding the project's budget and deadline. Thank you, and I look forward to the opportunity to collaborate on your project.
$350 USD in 3 days
2.3
2.3

Hi, We went through your project description and it seems like our team is a great fit for this job. We are an expert team which have many years of experience on Matlab and Mathematica, Statistics, Statistical Analysis, Data Science, Data Visualization, Data Analysis, Bioinformatics, Biostatistics Lets connect in chat so that We discuss further. Regards
$250 USD in 4 days
0.0
0.0

As a senior Full Stack Software Engineer cum experienced data analyst, I believe I am the perfect fit for your Spatial Transcriptomics Data Analysis project. With over a decade of experience developing voluminous AI/ML-powered systems, I have built robust solutions across various domains – including revolutionizing healthcare by integrating artificial intelligence in medical research and diagnostics. In terms of bioinformatics, I have a profound understanding of transcriptomics and its applications. Furthermore, my proficiency with Seurat ensures not only the successful integration of your specified cell markers and reference datasets but also the effective implementation of differential expression analysis, cell type identification, and spatial patterning analysis - aligning perfectly with your project's objectives. Building on my expertise in full stack engineering, you can rest assured that I'm well-equipped to handle the end-to-end responsibilities of your project. From ensuring HIPAA-compliant data security to utilizing AWS for efficient cloud storage and processing, I'll deploy all necessary measures to guarantee a reliable and scalable solution for your transcriptomic analysis needs.
$500 USD in 3 days
0.0
0.0

Hi, client ✋ I’m very interested in helping you analyze spatial transcriptomics data to uncover meaningful biological insights in lung disease. I have hands-on experience with Seurat for spatial transcriptomics, including differential expression, cell type annotation, and spatial pattern analysis. I will integrate your provided cell markers and reference datasets, perform robust DE analysis, identify cell populations, and analyze spatial organization across the three biopsies. The workflow will be reproducible, well-documented, and aligned with current best practices in spatial transcriptomics analysis. Best regards, Ihor H
$500 USD in 7 days
0.0
0.0

For your three samples: Preprocessing: QC, normalization (SCTransform), integration. Cell Type ID: Annotate using your markers and references (e.g., Human Lung Cell Atlas via SingleR). Differential Expression: FindMarkers with Wilcoxon/MAST, volcano plots, pathway analysis (clusterProfiler). Spatial Patterning: SpatialDE, Moran's I, ligand-receptor (CellChat), clustering (BayesSpace). Deliverables: Seurat object, RMarkdown report with code/figures, DE tables, spatial visualizations. Reproducible via GitHub. Strong bioinformatics/biostatistics background; publications in respiratory transcriptomics. Available now; completion in 7-10 days. Let's discuss details!
$250 USD in 9 days
0.0
0.0

Hi — I can analyze your spatial transcriptomics from the three lung biopsies in Seurat, covering QC + normalization/integration, cell type identification using your markers + reference mapping, differential expression, and spatial patterning (e.g., spatially variable genes and region/neighbor enrichment). I’ll use Seurat’s spatial workflow (SCTransform, anchors/transfer labels, SpatialFeaturePlot/SpatialDimPlot) and apply robust DE strategies (pseudobulk per biopsy where appropriate + cluster/region-level testing) to ensure results are biologically and statistically sound. I’ll deliver annotated cell-type maps, DE tables/figures, and spatial pattern summaries with reproducible scripts/notebooks—looking forward to collaborating and getting you publication-ready outputs.
$500 USD in 7 days
0.0
0.0

Hello, I can perform a full analysis of your spatial transcriptomics data from three lung biopsies using Seurat, integrating your specific cell markers and reference datasets. My expertise in bioinformatics, transcriptomics, and spatial data analysis ensures accurate and reproducible results. Scope of Work: Differential Expression Analysis: Identify genes with significant changes across conditions. Cell Type Identification: Annotate cell populations using your markers and reference datasets. Spatial Patterning Analysis: Map cell types and gene expression patterns across biopsy regions. Visualization: Generate clear plots and spatial maps for interpretation. Documentation: Provide a reproducible Seurat workflow with comments and a concise report of findings. You will receive the processed results, annotated datasets, and a documented pipeline so the analysis can be reproduced or extended in future studies. Best regards, Elaine López Marulanda AI‑Driven Solutions Architect | Bioinformatics Specialist
$750 USD in 7 days
0.0
0.0

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